By it's sexual organs.
how do you know if a tiger is a female or male?
Sex organs and generally the female is bigger in stature.
Only Tiger Two can answer this question.
Including the tail, an adult male tiger may measure some nine feet (3 meters) in length and weigh between 400 and 500 pounds (180 and 225 kilograms). The female is about a foot or so shorter and weighs about a hundred pounds (45 kilograms) less. Besides size, the full-grown male can be distinguished from the female by a ruff of long hair on the cheeks.